The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [human peripheral blood dataset]

Single-cell RNA-seq analysis of primary human peripheral blood mononuclear cells (PBMCs) from two independent B-ALL patients (Texas Children's Hospital) and healthy PBMC donor samples (STEMCELL Technologies). Single-cell RNA-sequencing libraries of primary human peripheral blood immune populations. B-ALL patient bone marrow samples were separated into CD19+ B cell and CD19-CD45+ non-B cell fractions by flow-based sorting and then mixed at a ratio of 1:5 CD19+:CD19-CD45+ followed by scRNA-Seq. Total CD45+ peripheral blood cells were sequenced from healthy donors.