RNA sequencing of mice primary Hepatic Stellate Cells with Exosomes from pancreatic cancer cells

Pancreatic ductal adenocarcinoma (PDAC) shows a remarkable predilection for liver metastasis. Prooncogenic secretome delivering and trafficking via exosomes is crucial for pre-metastatic microenvironment formation and metastasis. This study aims to explore the underlying molecular mechanisms of how PDAC derived exosomes (Pex) modulate the microenvironments of future sites of metastasis.So we dicided using Hepatic Stellate Cells to see their transcriptomes changing after Pex addition. Overall design: Examination of Mice primary Hepatic Stellate Cells RNA sequencing comparing the PDAC derived exosomes (adding Panc02/KPC derived exosomes) addition group with the control group (adding Exosomes from Primary mice pancreatic duct epithelial cells).