OrthoFinder analysis of nematode secretomes

The complex composition of parasite excretory/secretory proteins confounds the ability to identify those related to virulence and hence the success of an infection. Comparative analysis between ecologically diverse species may help prioritize proteins that play essential roles in parasitism for functional studies and may contribute to our understanding of the molecular basis of host-parasite specificity. The orthology analysis allowed to re-analyze all collected data simultaneously with the same tool, and resulted in a sequence-based clustering, independently of their initial annotation, reported in individual papers. We used the tool OrthoFinder (Emms DM, Kelly S. OrthoFinder: solving fundamental biases in whole genome comparisons dramatically improves orthogroup inference accuracy. Genome Biol. 2015 Aug 6;16:157).寄生虫排泄分泌蛋白（excretory/secretory proteins）的复杂组成，使得研究者难以鉴定与毒力相关、进而影响感染成功与否的蛋白。对生态多样的物种开展比较分析，有助于优先筛选出在寄生过程中发挥关键作用的蛋白以供功能研究使用，同时也能增进我们对宿主-寄生虫特异性分子基础的理解。直系同源分析（orthology analysis）允许我们使用同一工具同时对所有收集到的数据进行重新分析，最终得到基于序列的聚类结果，且该聚类不受单篇文献中报道的初始注释的限制。本研究使用了OrthoFinder工具（参考文献：Emms DM, Kelly S. OrthoFinder: 解决全基因组比较中的核心偏差可显著提升同源基因簇推断精度. Genome Biology, 2015年8月6日;16:157）。