LoopSeq Synthetic Long Read Sequencing Uncovers Isoform Modulation in the progression of Colon Cancer [LoopSeq]

In this study, we used a barcoding-based synthetic long read (SLR) isoform sequencing approach (LoopSeq) to generate sequencing reads sufficiently long and accurate to identify isoforms using standard short read Illumina sequencers. Loop transcriptome sequencing on 8 colon cancer samples. ID 1, 2 and 3 are for patient ID. N, T and M are for benign colon tissue adjacent to cancer samples, tumor samples and metastasis samples.