Rattus norvegicus genome sequencing

Chronic ethanol is known to affect the innate immune response of liver and suppress regeneration. In normal liver, regeneration occurs as a tightly orchestrated process involving many transcription factors that play key roles in the transcriptional regulation throughout the process. C/EBP-ß and C/EBP-a are two such regulators of liver growth and play complimentary roles in maintaining cellular differentiation, regulating metabolism and cell growth during liver regeneration. In this genome-wide binding study, we investigate the effect of chronic ethanol intake on the compensatory behavior of CEBP-ß and CEBP-a at baseline and followed by 70% partial hepectoctomy (PHx). We employed chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) to explore the ethanol-altered, genome-wide binding landscape of CEBP-ß and CEBP-a in rat liver post-PHx. We used a novel, dynamic comparative pattern count analysis to identify the effect of alcohol on altering the binding of CEBP-ß and CEBP-a post-PHx.