CIL:12996, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

A high resolution view of the tubulated CV membrane attached to ampullae 2, 3, 4 and 5 of the CVC. The tubules lie against the ribbon of microtubules as well as form a large mat lying inward (centrifically) from the ribbons. These tubular CV membranes are part of the smooth spongiome. TEM taken on 2/26/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X.The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).本图像为附着于CVC的2、3、4、5号壶腹的管状CV膜（tubulated contractile vacuole membrane）的高分辨率视图。这些小管既贴合微管带排布，又从微管带向内（向心性方向）形成一片大型膜垫。这类管状CV膜属于光滑海绵体（spongiome）的组成部分。本样品由R. Allen于1980年2月26日使用加速电压为75kV的日立HU11A型透射电子显微镜（Transmission Electron Microscopy, TEM）拍摄，放大倍数为22500倍。原始胶片采用爱普生Perfection V750 Pro扫描仪完成数字化扫描。该图像非常适用于定量分析工作。样品制备遵循标准流程：经戊二醛固定、四氧化锇后固定，乙醇梯度脱水，环氧树脂包埋，最终通过超薄切片机制备得到厚度约75nm的切片。更多详细信息可访问（http://www5.pbrc.hawaii.edu/allen/）获取。