AIMS Paint Rock Suspended Solids Data (AIMS_SE_PRF_TSSS)

This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 13 October 2021 and 13 February 2023, grab samples for Total Suspended Solids (TSS, mg/L) and seston Ash Free Dry Mass (AFDM, mg/L) were collected at the watershed outlet (PRM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2). Additional \"stretch\" samples were collected for TSS and AFDM at a tributary (PRB02) and downstream perennial monitoring site (PRP01), which are included in a separate file from the core TSS data from the Paint Rock research watershed. To measure TSS and AFDM concentrations, we collected 2- 3 replicates of unfiltered stream water in clean 1L Nalgene bottles after triple rinsing in the field, stored on ice, and brought to the lab for filtering. We brought samples back to the lab and filtered samples in 100mL batches through pre-ashed (550℃ for one hour) and pre-weighed 47 mm Whatman GF/F filters. Once the sample was filtered, the filters were placed in a drying oven for at least 48 hours and weighed. TSS was calculated as the (dry weight (filter + tin + sample) - dry weight (filter + tin)) / volume filtered (L). Ash free dry mass (AFDM) was then calculated as (dry weight (filter + tin + sample) - ashed weight (filter + tin + sample)) / volume filtered (L).本研究在美国阿拉巴马州杰克逊县的私人土地上的佩恩特罗克（Paint Rock）研究流域（出水口坐标：34.96861724，-86.16501705）开展。该流域汇水区域为一条汇入伯克斯溪（Burks Creek）的季节性无名支流，流域面积达2.97平方千米，地处坎伯兰高原（Cumberland Plateau）地貌区，植被以落叶阔叶林为主。研究流域位于阿拉巴马州埃斯蒂尔福克（Estillfork）附近，海拔范围介于211米至550米之间，是更大尺度田纳西河流域内佩恩特罗克河的支流。本区域属于湿润亚热带气候，1月与7月的日均气温分别为4.4℃与25.4℃，年平均降水量为1390毫米/年。 本次采样工作旨在支持“溪流微生物群水文间歇性效应（Aquatic Intermittency effects on Microbiomes in Streams, AIMS）”项目的核心采样目标。2021年10月13日至2023年2月13日期间，研究人员在流域出水口点位PRM01，于每3周一次的常规传感器维护期间采集总悬浮固体（Total Suspended Solids, TSS，单位mg/L）与浮游生物灰分干重（seston Ash Free Dry Mass, AFDM，单位mg/L）的瞬时水样（对应AIMS方法1）；同时在7个分布式长期监测点位开展季节性采样（对应AIMS方法2）。此外，研究人员还在支流点位PRB02与下游常年性监测点位PRP01采集了额外的“断面”水样用于TSS与AFDM检测，该部分样本数据收录于与佩恩特罗克研究流域核心TSS数据相互独立的单独文件中。 为测定TSS与AFDM浓度，研究人员先在野外用采样水预洗三次洁净的1L Nalgene塑料瓶，随后采集2-3组未过滤的溪流水样，置于冰上运输至实验室后进行过滤。将水样以100mL为单位，通过预先灼烧（550℃灼烧1小时）且预先称重的47mm Whatman GF/F滤膜进行过滤。过滤完成后，将滤膜置于干燥箱中至少烘干48小时后称重。总悬浮固体浓度计算公式为：（滤膜+锡箔+样品干重 - 滤膜+锡箔干重）/ 过滤体积（L）。灰分干重（AFDM）的计算公式则为：（滤膜+锡箔+样品干重 - 滤膜+锡箔+样品灰化后重量）/ 过滤体积（L）。