Anti-N antibody responses primed by neonatal nasal vaccination shifted from IgG1 to IgG2a and IgA isotypes when CpG were added as adjuvant.
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(a)Neonates were immunised i.n. at age 5–7 days as indicated (prime) and received one intranasal boost with N (10 µg) at 5 weeks. Sera and BALF were collected 7 days after the boost.(b)Antibody titers against N were determined by ELISA using endpoint dilution assay. Data are mean±SEM (≤30 or ≤3: not detected at the first tested dilution for sera or BALF).(c)Number of responders/number tested.
创建时间:
2015-12-02



