Dissociation temperatures of YscU<sub>C</sub> in presence of different divalent cations and <i>in vitro</i> binding affinities.
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CD spectroscopy at 220 nm was used to monitor thermal up- and down-scans of YscUC in presence of different divalent cations at a scan rate of 1°C/min to determine dissociation temperatures (Tdiss, compare Figure 3A). To measure the binding isotherms (Kd) of different divalent cations towards YscUC (compare Figure 3B) titrations monitored with CD spectroscopy at 220 nm were performed.
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2012-11-21
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