Metagenomic sequencing of gut microbiota associated with autism spectrum disorders patients

Here we report metagenomic sequencing data in gut microbiota of autism spectrum disorders (ASD) compared with healthy volunteers (30 for ASD children and 30 for healthy controls, respectively). The genes changed in autistic subjects involved 1,312,364 analytes that compare to 1,335,835 analytes in healthy controls. The number of taxa in autistic subjects were significantly increased as compared to the healthy controls based on the phylum and genus level (P = 0.001). However, the number of species were significantly decreased in autistic subjects (P = 0.001). Microbial DNA from fecal samples [healthy control (n=30), ASD patients (n=30)] were collected. A total amount of 1μg DNA per sample was used as input material for the DNA sample preparations. The DNA sample was fragmented by sonication to a size of 350 bp, then end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. Subject IDs (e.g. A3) in this study represent the same individual with identical id in GSE113690.