Latencies to pilocarpine effects and quantification of electrographic (ECoG) seizures preceding status epilepticus (SE).

Time intervals (min) to the onset of ECoG seizures and SE after the injection of pilocarpine are shown. The delay to SE onset after the first ECoG Racine's stage 4–5 seizure is also illustrated. JMV-1843 and ghrelin did not affect latencies to seizures or to SE onset compared to saline group (P>0.05; one-way ANOVA). JMV-1843 and ghrelin did not change the appearance of non-convulsive (stage 1–3) or convulsive seizures (stage 4–5) that preceded SE (P>0.05; one-way ANOVA). All rats pretreated with saline, ghrelin and JMV-1843 showed electrographic activity during behavioral stage 4–5 seizures and SE, while electrographic activity was often not detected by superficial electrodes during behavioral stage 1–3 seizures, most likely because activity was limited to deep structures.*t0 = pilocarpine injection.#t0 = first ECoG stage 4–5 seizure.