Immune crosstalk between pancreas, gut and liver through lymph node sharing [bulk RNA-seq]

To investigate tissue of origin effects on migratory dendritic cell (DC) gene expression in shared lymph nodes we performed bulk RNAseq of DCs from each tissue (liver, pancreas, duodenum). We then performed single cell sequencing of the DCs within the draining lymph nodes. Bulk RNAseq of FACs purified migratory dendritic cells from liver, pancreas and duodenum (n=4 each tissue, each tissue is 2 mice pooled) and single cell sequencing of YFP+ cells sorted from CD11c-YFP pancreatic-duodenal lymph nodes (n=3 each lymph node, each lymph node is 10 mice pooled).为探究组织起源对共同引流淋巴结内迁移性树突状细胞（dendritic cell, DC）基因表达的影响，我们首先对取自肝脏、胰腺及十二指肠的树突状细胞开展了批量RNA测序（bulk RNAseq）；随后对引流淋巴结内的树突状细胞实施了单细胞测序。本次研究包含两类测序数据：其一为经荧光激活细胞分选（fluorescence-activated cell sorting, FACS）纯化的、来自上述三种组织的迁移性树突状细胞的批量RNA测序，每个组织设置4个生物学重复，每个重复样本由2只小鼠的细胞混合制备；其二为从CD11c-YFP标记的胰十二指肠引流淋巴结中分选的黄色荧光蛋白（yellow fluorescent protein, YFP）阳性细胞的单细胞测序，每个淋巴结样本设置3个生物学重复，每个重复样本由10只小鼠的细胞混合制备。