Data underlying the publication: Species sorting shapes the divergence of a natural bacterial community with repeatable functionality during propagation with alternative substrates

This data is derived from a field based adaptive evolution experiment. Here, we explored a process of species sorting and its consequence on community-level functionality using mabisi, a traditional Zambian fermented milk, by exposing a common mabisi microbial community to five distinct substrates and propagating replicate communities at three farm sites in rural Zambia. The substrates - raw bovine milk (control), low-fat milk, full-cream milk, and the infant formulas F100 and S26 – represented novel environments, were propagated at a fixed dilution and static conditions to generate ~ 66 generations. Microbial community composition was profiled through 16S rDNA sequencing, while community-level functioning was assessed through volatile organic compound profiling by GC-MS, pH changes with a pH meter, and consistency analysis using an Adam's consistometer.

本数据集源自一项野外适应性进化实验。本研究以赞比亚传统发酵乳马比西（mabisi）为研究对象，将其中的常见微生物群落暴露于五种不同底物，并在赞比亚农村的三个农场位点对重复群落进行传代培养，以此探究物种分选过程及其对群落水平功能的影响。所用底物包括生鲜牛乳（对照组）、低脂牛乳、全脂牛乳以及婴儿配方奶粉F100与S26，均为全新的培养环境；实验以固定稀释率与静态培养条件开展传代，最终获得约66个世代的微生物群落。通过16S rDNA测序对微生物群落组成进行分析；群落水平功能则通过以下方式评估：采用气相色谱-质谱联用（GC-MS）进行挥发性有机化合物谱表征、借助pH计测定pH变化，以及使用亚当氏稠度仪（Adam's consistometer）开展稠度分析。