RNA-seq and anti-LMO2 ChIP-seq data in primary T cells in different stages from LMO2 over-expressing C57BL/6J transgenic mice

In this study, to investigate the pathogenic role of transcriptional regulator LMO2 during T lineage development, we isolated DN1, DN3, DP, CD4SP, CD8SP thymocytes, splenic CD4+ T cells and splenic CD8+ T cells from wild type and LMO2 over-expressing C57BL/6J mice for RNA-seq, and DN3 (CD25+), DP thymocytes, splenic CD4+/CD8+ T cells from transgenic mice and wild type DN3 (CD25+) thymocytes for ChIP-seq. Overall design: paired samples in same stages (DN1, DN3, DP…) from WT and TG mice were subjected to RNA-seq to dected the difference of transcriptome and DN3 (CD25+), DP thymocytes, splenic CD4+/CD8+ T cells from transgenic mice and wild type DN3 (CD25+) thymocytes were subjected to ChIP-seq to detect the DNA-binding feature of LMO2.