Bulk RNA-seq of aorta intima resident macrophage from B6, and adventitia macrophages from ApoE-/- mice

Macrophages in atherosclerotic aorta are major population in lesion and contributes to lesion formation by becoming foam cells. To investigate in determine diversity of arterial macrophage, we extracted aorta intima resident macrophages (CD11c+ MHC II+ CD64+ macrophages from C57BL/6 mice, as well as adventitia macrophages from atherosclerotic aorta (which complement our prior submissions of foamy and non-foamy intima macrophage). Our data identify unique gene expression signatures for these different macrophage populations in the aorta. C57BL/6 samples were derived from 5-mice pooled for each sample. Adventitia macrophage samples from ApoE-/- mice (n=6) were fed high fat diet for 28 weeks prior to sacrifice. Samples were sorted on a BD Aria III and sequenced by Illumina HiSeq-3000.