Liver-specific overexpression of GBP5 induces liver injury and inflammation

Purpose: The goal of this study is to compare transcriptome profilings of livers in which GBP5 and ßGal were overexpressed, respectively. Methods: Liver-specific GBP5-overexpressing and ßGal–overexpressing mice were generated by tail-vein injection of individual purified adenovirus(6 × 1011 viral particles per mouse), respectively. RNA-seq was performed by using HiSeq X Ten platform in a single test. Paired-end clean reads were aligned to the mouse reference genome (Ensemble_GRCm38.89) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1). Conclusion: Our study represents the first detailed analysis of liver transcriptomes from GBP5- and ßGal-overexpressing livers, generated by RNA-seq technology. Our results show that 633 genes were significantly up-regulated in the GBP5-overexpressing livers, whereas 586 genes were dramatically down-regulated in the GBP5-overexpressing livers. GO analysis showed that some up-regulated genes were related to the immune system process, response to stress, cell cycle, and cell death in GBP5-overexpressing livers. Overall design: mRNA profiles of ßGal- and GBP5-overexpressing livers were generated by deep sequencing, in a single test, using HiSeq X Ten platform.