Effect of high glucose on transcriptomic expression of cholangiocarcinoma cells

This project was aimed to study the transciptomic profiles of cholangiocarcinoma cells cultured in different concentration of glucose. The established human cholangiocarcinoma cell line; KKU-213, and highly metastatic subline; KKU-213L5, were used. KKU-213 were cultured in either Dulbecco Modified Eagle's Medium (DMEM) with normal (5.6 mM) or high glucose (25 mM) and labeled as KKU-213NG or KKU-213HG according to thier cuture medium and KKU-213L5 was cultured in high glucose DMEM medium. The RNA from all conditions (KKU-213NG, KKU-213HG and KKU-213L5) were then extracted and quality-controlled. Qualified RNA were subjected to sequenced and transcriptomics were processed for the abundance of genes expresed in each condition. The differentially expressed genes were then selected for furhter study to figure out the interaction between them and also the candidate genes that might be a promising target for the treatment of cholangiocarcinoma patients who also have diabetes mellitus. Comparative transcritptomic expression between cholangiocarcinoma cell lines cultured in normal or high glucose medium.