Ureaplasma urealyticum 4155 Genome sequencing and assembly. Ureaplasma urealyticum 4155

Isolated from placenta on 3/24/1989 in an intrauterine growth restriction study done at the University of Alabama at Birmingham. This patient isolate could not be serotyped using real time PCR assays designed to distinguish among the 14 ATCC type strains for the 14 known ureaplasma serovars (2, 3). It gave positive results for several serovars even after filter cloning. Comparison of the isolate 4155 partial genome sequence with 18 other completely or partially sequenced genomes showed extensive horizontal gene transfer among the different isolates (1) 1. Paralanov, V., J. Lu, L. Duffy, D. Crabb, L. Xiao, J. A. Robertson, B. A. Methe, G. H. Cassell, K. B. Waites, and J. I. Glass. 2012. Comparative Genome Analysis of 19 Ureaplasma urealyticum and Ureaplasma parvum strains. BMC Microbiology, in press. 2. Xiao, L., J. I. Glass, V. Paralanov, S. Yooseph, G. H. Cassell, L. B. Duffy, and K. B. Waites. 2010. Detection and characterization of human Ureaplasma species and serovars by real-time PCR. J Clin Microbiol 48:2715-2723. 3. Xiao, L., V. Paralanov, J. I. Glass, L. B. Duffy, J. A. Robertson, G. H. Cassell, Y. Chen, and K. B. Waites. 2011. Extensive horizontal gene transfer in ureaplasmas from humans questions the utility of serotyping for diagnostic purposes. J Clin Microbiol 49:2818-2826.