The transcriptional regulator LysG of Mycobacterium tuberculosis activates lysE in a lysine-dependent manner

To evaluate what genes are under the transcriptional control of LysG(Mt), a whole genome differential gene expression analysis of Wt Mt (Wt_Mtb) and del_ysG(1985c) was done, based on RNA-seq. Both were grown in a minimal medium supplemented with either 5 mM lysine, 10 mM ammonium or 3.34 mM histidine as sole nitrogen source. TruSeq Stranded mRNA Library Prep Kit from (Illumina, (San Diego, CA, USA) was used to prepare the cDNA libraries to analyze the whole transcriptome. An additional library was prepared from a pool of all isolated RNA according to the protocol of Pfeifer-Sancar et al. focusing on native 5'-ends of transcripts.