Peptides tested in this study for their crystallin aggregation properties.

Water-soluble extract (2 mg) from 35-year-old human lens was incubated in 50 mM phosphate buffer, pH 7.2 at 37°C for 24 h in the presence or absence of various peptides (25 µg). The precipitate formed was collected by centrifugation at 1000× g for 10 min and dissolved in buffer containing 7 M urea and the amount of protein was estimated by Bio-Rad protein assay method. The values are an average of two independent experiments. Lens extract alone showed the precipitation of ∼12 µg of proteins after 24 h of incubation. Bold letters in sequence represent substitutions.