Dataset of the bacterial fitness estimation values and mutation identifications from: Rapid decline of adaptation of Pseudomonas fluorescens to soil biotic environment

Interactions between microbes can both constrain and enhance adaptation, but to date, most studies employ simplified communities and environments. We measured fitness of populations of the soil bacterium Pseudomonas fluorescens that have been evolved in both the presence and absence of a natural potting soil microbial community. Populations from both environments showed similar fitness increases with respect to the ancestor in the absence of the community, suggesting no significant cost of evolving with the community. By contrast, fitness in the presence of the community increased for community-evolved populations, but decreased considerably below the ancestral state for populations evolved in the absence of the community. This suggests some abiotic beneficial mutations are costly in the presence of the community, while others are not, with the former only selected against in the presence of the community. Most mutations underpinning fitness changes were clone specific, supporting the v...微生物间的相互作用既可制约又可促进适应性演化，但迄今为止，绝大多数相关研究均采用简化的群落与实验环境。我们针对土壤细菌荧光假单胞菌（Pseudomonas fluorescens）的种群展开实验：分别在有天然盆栽土壤微生物群落存在与不存在的两种条件下对其进行演化培养，并测定了各种群的适应度（fitness）。在无微生物群落的条件下，两种演化环境所得的种群相对于其祖先菌株均呈现出相似的适应度提升，这表明在有微生物群落的条件下进行演化并未带来显著的适应度代价。与之形成鲜明对比的是，在有微生物群落存在的条件下，经群落共演化的种群适应度有所提升，而在无群落环境中演化的种群其适应度则大幅下降至祖先菌株的适应度以下。这表明部分非生物有益突变在微生物群落存在的条件下会产生适应度代价，而另一部分则不会；仅当群落存在时，前者才会受到负选择。介导适应度变化的绝大多数突变为克隆特异性突变，这支持了……