Biosolid data of Dry Sanitation System from Berriozabal, Chiapas, Mexico

The dry sanitation system (DSS) is an alternative eco-technique for water care in marginalized communities where water resources are limited. Additionally, the biosolids generated can be used for soil restoration and agricultural purposes. In order to reuse these biosolids, the presence and quantity of fecal coliform bacteria must be determined. The amount of coliforms found in a sample can be used to assess unhealthiness of biosolids from DSS. Within fecal coliforms, E. coli is classified into different pathotypes according to its pathogenicity mechanism such as ETEC, EPEC, EIEC, STEC, EAEC and DAEC. In this study, biosolids from DSS at Berriozabal, Chiapas, Mexico, were analyzed for a year. Fecal coliforms were measured with the most probable number technique, while E. coli pathotypes were investigated by PCR. Fecal coliforms were isolated from 50% of the biosolids simples and two strains of E. coli with the bfpA virulence gene of the enteropathogenic E. coli (EPEC) pathotype were isolated. Most of the biosolids obtained from DSS can be used as compost for ornamental plants. These analyzes are important to determine the presence of coliforms in biosolids and propose strategies that reduce this type of microorganisms in biosolids generated with this eco-technique, so that they can be used for agricultural purposes in these marginalized communities of Chiapas.干式卫生系统（Dry Sanitation System，DSS）是水资源匮乏边缘化社区中用于水资源管护的替代性生态技术。此外，其产生的生物固体可用于土壤修复与农业生产。为实现此类生物固体的资源化利用，需对粪便大肠菌群的存在情况与数量进行检测。样本中检出的大肠菌群数量可用于评估干式卫生系统所产生物固体的卫生安全风险。在粪便大肠菌群中，大肠杆菌（Escherichia coli，E. coli）可根据其致病机制划分为不同致病型，如产肠毒素大肠杆菌（Enterotoxigenic Escherichia coli，ETEC）、肠致病性大肠杆菌（Enteropathogenic Escherichia coli，EPEC）、肠侵袭性大肠杆菌（Enteroinvasive Escherichia coli，EIEC）、产志贺毒素大肠杆菌（Shiga toxin-producing Escherichia coli，STEC）、肠聚集性大肠杆菌（Enteroaggregative Escherichia coli，EAEC）与弥散黏附性大肠杆菌（Diffusely adherent Escherichia coli，DAEC）。本研究对墨西哥恰帕斯州贝里奥萨瓦尔地区的干式卫生系统所产生物固体开展了为期一年的检测分析，采用最大似然数法检测粪便大肠菌群，通过聚合酶链式反应（Polymerase Chain Reaction，PCR）分析大肠杆菌致病型。结果显示，50%的生物固体样本中检出粪便大肠菌群，同时分离得到两株携带肠致病性大肠杆菌（EPEC）致病型bfpA毒力基因的大肠杆菌。多数干式卫生系统所产生物固体可作为堆肥用于观赏植物栽培。本研究相关检测对于明确生物固体中的大肠菌群存在情况、制定针对该生态技术所产生物固体的微生物消减策略具有重要意义，进而可助力恰帕斯州此类边缘化社区将其应用于农业生产。