circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1. Homo sapiens

To identify the circNEIL3-interacting protein, we performed RNA pull-down assays after transfecting into cells with biotin-labeled sense (S) or antisense (AS) DNA oligomers crossing the junction site of circNEIL3, and the pull-down proteins were subjected to SDS-PAGE and Coomassie blue staining. Band ~45 kDa were pulled down by the AS probe consistently from MDA-MB-231 cell line, and it was specifically cut off and used for mass spectrometry analysis.