Gene knockouts (KOs) across 4 Saccharomyces cerevisiae strains made with two methods are sequenced in their whole genome sequences

Loss-of-function (LoF) mutations associated with disease don't manifest equally in different individuals, a phenomenon known as incomplete penetrance. The impact of the genetic background on incomplete penetrance remains poorly characterized. Here, we systematically assessed the changes in gene deletion phenotypes for 3,786 gene knockouts in four Saccharomyces cerevisiae strains and 38 conditions. A number of KOs made with two distinct methods across the four genetic backgrounds are here sequenced and compared.