Identification of Angustifolia 3 (ZmAN3) target genes through GSyellow-based ChIP-seq in Zea mays leaves. Identification of Angustifolia 3 (ZmAN3) target genes through GSyellow-based ChIP-seq in Zea mays leaves

Genome-wide target genes of ZmAN3 were identified through ChIP-seq on the growth zone of the maize leaf, encompassing the division, transition and expansion zone. For ChIP-seq, ZmAN3 was fused to the GSyellow TAP tag and expressed from the ubiquitin promoter (pUBIL). The pUBIL:ZmAN3-GSyellow construct was transformed into the maize inbred line B104. ChIP was performed using anti-GFP antibody (abcam290).