Description and definition of 15 central complex related events used in this study to illustrate heterochronic development in Tribolium castaneum (Tc), Drosophila melanogaster (Dm) and Schistocerca gregaria (Sg).

Events were defined by using our dataset of anti-GFP and anti-synapsin stainings with both species, to determine potential differences between them, and by using the central complex literature as reference point. Time points for each event are included, as absolute time in hours and relative time per developmental period in percent. Note that length of embryonic developmental periods was taken from [37,68] and Scholten and Klingler (unpublished), stages were determined using morphological criteria and then time points were calculated from these works. Larval and pupal developmental times were determined specifically for our rx transgenic lines and pupal time points were then verified by morphological criteria using works by Dippel (unpublished) and [92]. Information on Schistocerca central complex events as well as relative and absolute developmental time was taken from [7,33,80,81]. CB, central body, Dm, Drosophila melanogaster; EB, ellipsoid body; FB, fan-shaped body; NO noduli; PB, protocerebral bridge; Sg, Schistocerca gregaria; Tc, Tribolium castaneum. (XLSX)