Construction of genetic linkage map based on SNP markers, QTL mapping and detection of candidate genes of growth-related traits in Pacific abalone using genotyping-by-sequencing

Pacific abalone (Haliotis discus hannai) is a commercially important high valued molluscan species. Its wild population has decreased in recent years. Pacific abalone is widely cultured in Korea. Traditional breeding programs have been implemented for hatchery production of abalone seeds. To obtain more genetic information for the molecular breeding program, a high-density linkage map and quantitative trait locus (QTL) for three growth-related traits was constructed for Pacific abalone. F1 cross population with two parents were sampled to construct the linkage map using genotyping by sequencing (GBS). A total of 664,630,534 clean reads and 56,686 SNPs were generated. In sum, 3,345 segregating SNPs were used to construct a consensus linkage map. The map spanned 1,747.023 cM with 18 linkage groups and an average interval of 0.55 cM. QTL analysis revealed two significant QTL in LG10 on the consensus linkage map in each growth-related trait. Both the QTLs are located in the telomere region ...太平洋鲍（*Haliotis discus hannai*）是一种兼具重要商业价值与高经济价值的软体动物。近年来其野生种群数量持续下降。该物种在韩国被广泛养殖，目前已针对鲍鱼苗种的工厂化繁育建立了成熟的传统育种程序。为获取分子育种所需的更多遗传信息，研究人员针对太平洋鲍构建了高密度连锁图谱，并开展了3个生长相关性状的数量性状位点（quantitative trait locus，QTL）定位分析。本研究以双亲杂交获得的F1代群体为实验材料，采用测序基因分型（genotyping by sequencing，GBS）技术构建连锁图谱。最终共得到664,630,534条洁净读段（clean reads）与56,686个单核苷酸多态性（single nucleotide polymorphism，SNP）位点。经筛选后，最终选取3,345个分离性SNP位点用于构建整合连锁图谱。该图谱总遗传距离达1,747.023 cM，包含18个连锁群，平均标记间隔为0.55 cM。QTL分析显示，在整合连锁图谱的LG10连锁群上，针对各生长相关性状均检测到2个显著关联的QTL位点，且二者均位于端粒区域……