Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.
收藏PubMed Central1991-03-15 更新2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC51246/
下载链接
链接失效反馈官方服务：
资源简介：
Combinatorial libraries of antibody heavy and light chains derived from the peripheral blood lymphocytes of an individual immunized with tetanus toxoid have been expressed in Escherichia coli by using phage lambda vectors. Screening of the libraries allowed identification of a large number of human monoclonal Fab fragments specific for tetanus toxoid. Initial studies suggested considerable sequence diversity in these antibodies. The method should allow the generation of many human monoclonal antibodies of interest and the dissection of human humoral immune responses. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-03-15
