Single cell analysis of iNKT cells from murine epididymal adipose tissue and spleen

We confirm previous findings that adipose iNKT cells are transcriptionally distinct from canoncial splenic iNKT cells and we demonstrate that the adipose iNKT cell population is hetergeneous, containing two major functional subsets which can be segregated by expression of the surface marker NK1.1 (Klrb1c). Both of these adipose iNKT cell subsets play a role in the regulation of adipose tissue tissue homeostasis, including NK1.1+ iNKT cells, which produce IFNγ and induce adipose NK cell-mediated killing of adipose tissue macrophages. Unbiased single cell sequencing of sorted whole murine iNKT cells from epidiymal adipose tissue (one sample) and spleen (two samples hashed together, one sample from untreated mice and one sample from mice treated with 1μg aGalactosylCeramide)