Impact of the chemical composition of applied organic materials on bacterial and archaeal community compositions in paddy soil

Microcosm experiments were performed to investigate the effect of organic material composition on soil bacterial and archaeal communities under paddy soil conditions. Rice straw (RS) and farmyard manure (FM) were used as the original organic materials, and three types of residues were prepared by sequential extraction from the original materials (R1RS and R1FM, lipid extracted organic materials; R2RS and R2FM lipid and sugar extracted organic materials; R3RS and R3FM, lipid, sugar, and hemicellulose extracted materials). The organic materials were applied to soil microcosms at a 5 or 10 Mg/ha level. Soil microcosms were incubated under flooded conditions, and DNA was extracted at 0, 1, 2, 4, 8, and 16 weeks after the incubation.