Poly(A)-ClickSeq resolves CF25-mediated alternative poly-adenylation, HeLa. Homo sapiens
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下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA374982
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资源简介：
Poly(A)-ClickSeq is a new methodology for the sequencing of the 3'UTR/poly(A) tail junction of RNA. We analysed both wild-type and CF25Im knock-down HeLa cells in culture using Poly(A)-ClickSeq to find the distribution of poly(A) sites in these samples and determine the role of CF25Im in poly(A) site selection (alternative poly-adenylation, APA). Overall design: RNA was extracted from HeLa cells with or without CF25Im Knock-down, each in triplicates, and sequenced using Poly(A)-ClickSeq and sequencing once on a HiSeq and once on a MiSeq
创建时间：
2017-02-15
