Gene Expression Changes Using Neurotransmitter Receptors and Regulators PCR Array: Chronic Morphine vs. Saline Control Groups.

Gene Expression Changes in Ventral and Ventrolateral Periaqueductal Gray with Chronic Morphine Treatment at the level of Inferior Colliculus (for anatomical location of region of analysis, see Fig. 1). Out of 84 total genes in the Neurotransmitter Receptors and Regulators PCR Array (SABiosciences, MD), 4 genes showed statistically significant change (pt-test). Total of 5 housekeeping genes were used as a reference and showed no changes between treatments. These included: Actb, Hprt1, Ldha, Rpl13a, Rplp1. Gene nomenclature was adopted from SABiosciences and corresponds to PubMed. Abbreviations: Abat, 4-aminobutyrate aminotransferase; AchE, acetylcholinesterase; Anxa9, annexin A9;Brs3, Bombesin-like receptor 3; Cckar, cholecystokinin A receptor; Cckbr, cholecystokinin B receptor; Chat, choline acetyltransferase; Chrm1–5, cholinergic receptor, muscarinic 1–5 (muscle); Chrna1–6, cholinergic receptor, nicotinic, alpha 1–6 (neuronal); Chrnb1–4, cholinergic receptor, nicotinic, beta 1; Chrnd, cholinergic receptor, nicotinic, delta; Chrne, cholinergic receptor, nicotinic, epsilon; Chrng, cholinergic receptor, nicotinic, gamma; Comt, catechol-O-methyltransferase; Drd1a, dopamine receptor D1A; Drd2–5, dopamine receptor D2–5; Gabra1–6, gamma-aminobutyric acid (GABA) A receptor, alpha 1–6; Gabrb2–3, gamma-aminobutyric acid (GABA) A receptor, beta 2–3; Gabrd, gamma-aminobutyric acid (GABA) A receptor, delta; Gabre, gamma-aminobutyric acid (GABA) A receptor, epsilon; Gabrg1–2, gamma-aminobutyric acid (GABA) A receptor, gamma 1–1; Gabrp, gamma-aminobutyric acid (GABA) A receptor, pi; Gabrq, gamma-aminobutyric acid (GABA) A receptor, theta; Gabrr1–2, gamma-aminobutyric acid (GABA) A receptor, rho 1–2; Gad1–2, glutamate decarboxylase 1–2; Galr1–3, galanin receptor 1–3; Glra1–3, glycine receptor, alpha 1–3; Glrb, glycine receptor, beta; Grpr, gastrin releasing peptide receptor; Htr3a, 5-hydroxytryptamine (serotonin) receptor 3a; Maoa, monoamine oxidase A; Mc2r, melanocortin 2 receptor; Nmur1–2, neuromedin U receptor 1–2; Npffr1–2, neuropeptides FF receptor1–2; Npy1r-2r, 5r, neuropeptide Y receptor 1–2,5; Ppyr1, pancreatic polypeptide receptor 1; Prima1, proline rich membrane anchor 1; Prlhr, prolactin releasing hormone receptor; Prokr1–2, prokineticin receptor 1–2; Qrfpr, proglutamylated RFamide peptide receptor; Slc5a7, solute carrier family 5 (choline transporter) member 7; Sstr1–5, somatostatin receptor 1–5; Tacr1–3, tachykinin receptor 1–3; Th, tyrosine hydroxylase.