The recent publication showed the DNA-mediated stack formation of nanodiscs. Background: Membrane-scaffolding proteins (MSPs) become a versatile tool in generating nano-sized discoidal membrane mimeti
A problem rarely tackled by current DNA assembly methods is the issue of cloning additional parts into an already assembled construct. Costly PCR workflows are often hindered by repeated sequences, an
Herein we propose a fully in vitro iterative method based on Golden Gate Aseembly to faithfully construct a long synthetic DNA molecule starting from commercially available double-stranded DNA. The sy
Algorithms and information, fundamental to technological and biological organization, are also an essential aspect of many elementary physical phenomena, such as molecular self-assembly. Here we repor
