Human Protein Atlas - Cell Atlas

The Cell Atlas provides high-resolution insights into the expression and spatio-temporal distribution of RNA and proteins in human cell lines. Genome-wide mRNA expression is determined by deep RNA-sequencing in a panel of 69 cell lines, representing various cell populations in different organs and tissues of the human body. The subcellular distributions of proteins encoded by 12813 genes (65% of the human protein-coding genes) are investigated in a subset of cell lines selected based on corresponding RNA expression. Protein localization data is derived from antibody-based profiling, using immunofluorescence (ICC-IF) and confocal microscopy, and classified into 35 different organelles and fine subcellular structures. The Cell Atlas offers a database for detailed exploration of individual genes and proteins of interest, as well as for systematic analysis of transcriptomes and proteomes in broader contexts, in order to increase the understanding of human biology at the cellular and subcellular levels.

细胞图谱提供了对人类细胞系中RNA和蛋白质表达及其空间-时间分布的高分辨率洞察。通过对69个细胞系进行全基因组mRNA表达深度RNA测序，确定了代表人体不同器官和组织中不同细胞群体的细胞系的全基因组mRNA表达。在基于相应RNA表达选择的细胞系子集中，研究了编码于12813个基因（占人类蛋白质编码基因的65%）的蛋白质在亚细胞水平上的分布。蛋白质定位数据通过基于抗体的分析获得，利用免疫荧光（ICC-IF）和共聚焦显微镜技术，并将其归类于35种不同的细胞器和精细的亚细胞结构。细胞图谱提供了一个数据库，用于详细探索感兴趣的个体基因和蛋白质，以及系统分析更广泛背景下的转录组和蛋白质组，以增进对细胞和亚细胞水平上人类生物学的理解。