Environmentally-driven holobiont changes impact thermotolerance for Tropical Eastern Pacific corals

Our study's goal is to investigate how seasonal upwelling in Panama's Tropical Eastern Pacific influences host-microbiome configurations in Pocillopora spp. corals and test the ability of holobionts to resist increasing water temperatures. To do so, we engaged in shallow shotgun sequencing to characterize the host's population genetics, and ITS2 and 16S rRNA marker gene sequence to characterize coral's algal Symbiodiniaceae and prokaryotic microbiome members, respectively.