Ongoing Production of Tissue-Resident Macrophages from Hematopoietic Stem Cells in Healthy Adult Macaques [ATAC-seq]

To understand the ontogeny and longevity of tissue-resident macrophages in nonhuman primates (NHPs), we employ a model of autologous hematopoietic stem progenitor cell (HSPC) transplantation with HSPCs genetically modified to be marked with clonal barcodes, allowing for subsequent analysis of clonal ontogeny. Overall design: Macrophages from animals that had been transplanted with barcode and GFP labeled stem cells were compared with non-GFP macrophages and macrophages from control, unmanipulated animals.