JV4404 is deletion of the Dot.Icm system. Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in
These are H. pylori cells that don't have a complete FliP. In addition, FliO was knocked out in . these cells. The aim is to study the assembly of the flagellar motor and the core complex.
