Research data for paper Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS

Research data for paper Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS (FASEB Journal October 2022).

This dataset consists of two Excel spreadsheets containing molecular biological and electrophysiological data, respectively.

The data used for Figure 2 was obtained by measuring the expression levels of the CBP mRNA in different tissues of Lymnaea by using qRT-PCR. The data used for the graph and associated statistical analysis show that the expression level is highest in the central nervous system (CNS).

The data used for Figure 6F was obtained by intracellular microelectrophysiology to measure the amplitude of the monosynaptic EPSP evoked in the motoneuron B1 by the electrical stimulation of the Cerebral Giant Cell (CGC) under different experimental conditions. The data used for the graph and associated statistical analysis show that injection of cAMP into the CGC cell body increased EPSP amplitude in the B1 motoneuron, but pre-treatment with either of the three HAT inhibitors prevented the cAMP-induced increase of EPSP. It also shows that 5'AMP that was used as a control for cAMP did not increase the amplitude of the B1 EPSP.