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收藏DataCite Commons2025-10-10 更新2026-05-05 收录
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https://data.scielo.org/file.xhtml?persistentId=doi:10.48331/SCIELODATA.GANUQS/Z1ABSR
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资源简介：
Macrophage polarization and flow cytometry analysis. CD14⁺ monocytes were cultured in vitro and differentiated into M1 or M2 macrophages. Following an initial 24-hour incubation, M1 polarization was induced by treatment with lipopolysaccharide (LPS) and interferon-gamma (IFN-γ), whereas M2 polarization was achieved by stimulation with interleukin-4 (IL-4) and interleukin-13 (IL-13) for an additional 48 hours. Cells were harvested, gated on CD68⁺ macrophages, and analyzed by flow cytometry for surface markers HLA-DR, CD206, and CD86 to confirm polarization states.
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SciELO Data
创建时间：
2025-10-09
