Antibody Cocktail Freezing - Storage conditions

Test whether antibody cocktails for mass cytometry can be preserved for long times. Test which storage conditions of antibody cocktails serve best for this purpose. Test whether different metal tags are compatible with long-term storage.
Conclusion:
The overall antibody staining pattern and frequency distribution were comparable to freshly prepared antibody mixtures and remained constant even after storage for up to 19 months in LN2. Also median signal intensities of the 11 antibody conjugates were preserved or even slightly higher in the frozen cocktails, negating any storage time-dependent decrease of signals. The data further demonstrated the compatibility of cocktail freezing with antibody conjugates generated by different labeling techniques, i.e. lanthanide-, palladium- and platinum-antibody conjugates, and mixtures thereof.
Notes:
Refers to Fig 1B-D of the manuscript EQ 4 element normalization beads Data is already normalized (Fluidigm norm algorithm)