Location of target sequences within specific topological zones relative to the NM.

Location of the specific gene-target sequences in the topological zones relative to the NM. (D) distal to NM, (P) proximal to NM, (VC) very close to NM and (E) embedded within NM. Note that in P0 neurons most of the gene sequences were located in the embedded zone, while in the old neurons (P540) most of the gene sequences were located either in the proximal or distal zone relative to the NM. New-born (P0), 7-day-old (P7), adult (P80), old (P540) rats. (NFM) neurofilament medium; (NFL) neurofilament light; (MBP) myelin basic protein; (GFAP) glial fibrillary acidic protein; (MPZ5′) myelin protein zero region 5′; (MPZ3′) Myelin protein zero region 3′; (ACT) β-actin; (ALB) albumin; (AFP) α-fetoprotein. ± indicates that in one experiment the amplification of the target sequence was negative in the sample corresponding to the topological zone indicated (n≥4).