Exploration of resistance mechanisms in Gram-negative and Gram-positive organisms from Suva, Fiji

We collected a set of Gram-negative and Gram-positive clinical isolates between July 2020 - October 2022 from Colonial War Memorial Hospital in Suva, Fiji. We sampled both sensitive and resistant isolates, and only bacterial isolates belonging to the following taxonomic groups were targeted for inclusion: Acinetobacter, Enterobacterales (excluding Salmonella and Shigella), Pseudomonas, Staphylococcus aureus or Enterococcus.in brief, target Gram-negative organisms that were from blood and/or carbapenem resistant were prioritised, with a random sampling of carbapenem susceptible isolates from other sites. Gram-negative isolates were classified as third-generation cephalosporin resistant (3GCR) if there was evidence of either ESBL production or resistance to at least one third-generation or fourth-generation cephalosporin (ceftriaxone, ceftazidime, cefepime). For S. aureus, blood isolates were prioritised, followed by a random sampling of methicillin-susceptible and resistant organisms from other sites. Finally, each identified VRE isolate was matched with two vancomycin-susceptible isolates to provide comparisons.