Ebf3 knockdown cells show characteristic irregular migration, leading to the inhibition of CP invasion

We performed in utero electroporation at E14.5, and examined neuronal migration and morphology after 48 h. Subsequent time-lapse imaging analyses revealed striking differences in migration profiles between control and Ebf3 loss-of-function cells.本研究于胚胎第14.5天（E14.5）开展子宫内电穿孔（in utero electroporation）实验，并在48小时后对神经元迁移与细胞形态进行观测分析。后续延时成像（time-lapse imaging）分析结果显示，对照组与Ebf3功能缺失（loss-of-function）细胞的迁移特征存在显著差异。