Figure S1 - Myocardial Injection of Apelin-Overexpressing Bone Marrow Cells Improves Cardiac Repair via Upregulation of Sirt3 after Myocardial Infarction

Overexpression of apelin reduces apoptosis and increases proliferation of cultured BMCs. A. Representative images and quantification of serum-free (starvation) induced cell apoptosis in cultured BMCs isolated from WT and Sirt3 KO mice transfected with Ad-apelin and Ad-GFP. Transfected of BMCs with Ad-apelin attenuated starvation-induced cell apoptosis. Starvation-induced cell apoptosis was significantly increased in cultured BMCs of Sirt3KO mice transfected with Ad-apelin compared to that of WT mice transfected with Ad-apelin (n = 3 mice, *p<0.05). B. Transfected of BMCs with Ad-apelin significantly increased cell proliferation compared to GFP transfected BMCs. The proliferative rate of BMCs was significantly reduced in cultured BMCs of Sirt3KO mice compared to that of WT mice transfected with Ad-apelin as measured by MTT method (n = 3 mice, *p<0.05).
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