E. faecium MurAA in complex with UDP-N-acetylmuramic acid (UNAM) and a covalent adduct of PEP with Cys119

E. faecium MurAA in complex with UDP-N-acetylmuramic acid (UNAM) and a covalent adduct of PEP with Cys119 Descriptor: (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid, UDP-N-acetylglucosamine 1-carboxyvinyltransferase Authors: Zhou, Y, Shamoo, Y. Deposit date: 2022-06-07 Release date: 2023-07-05 Last modified: 2025-01-15 Method: X-RAY DIFFRACTION (2.65 Å) Cite: Enolpyruvate transferase MurAA A149E , identified during adaptation of Enterococcus faecium to daptomycin, increases stability of MurAA-MurG interaction. J.Biol.Chem., 299, 2023粪肠球菌（Enterococcus faecium, E. faecium）的MurAA蛋白，与UDP-N-乙酰胞壁酸（UDP-N-acetylmuramic acid, UNAM）以及磷酸烯醇式丙酮酸（phosphoenolpyruvate, PEP）与Cys119形成的共价加合物所构成的复合物。配体描述：(2R)-2-{[(2R,3R,4R,5S,6R)-3-(乙酰氨基)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-二氧代-3,4-二氢嘧啶-1(2H)-基)-3,4-二羟基四氢呋喃-2-基]甲氧基}(羟基)磷酰基]氧基}(羟基)磷酰基]氧基}-5-羟基-6-(羟甲基)四氢-2H-吡喃-4-基]氧基}丙酸。作者：Zhou, Y.、Shamoo, Y.。提交日期：2022年6月7日；发布日期：2023年7月5日；最后修改日期：2025年1月15日。实验方法：X射线衍射法（分辨率2.65 Å）。引用文献：《在粪肠球菌适应达托霉素过程中鉴定得到的烯醇式丙酮酸转移酶MurAA A149E可增强MurAA与MurG的相互作用稳定性》，《生物化学杂志》（J. Biol. Chem.），299卷，2023年。