Analysis of Ifi27l2a-LacZ reporter gene expression by flow cytometry.

Lungs from infected and non-infected KO mice were harvested and homogenized as described in material and methods. After staining with specific antibodies for macrophages, B cells, T cells and NK cells the respective cell populations were analyzed for expression of the LacZ reporter gene using a fluorescent substrate (fluorescein di β-D-galactopyranoside, see material and methods for details). Leuko %: percentage of respective cell population (CD11bhigh, CD19, CD3 and NKp46) with respect to total leukocyte counts. Please note that the total percentages do not add up to 100% of the leucocytes since there are also immune cells that do not express any of the respective markers. FLuoRep+ %: percent of cells positive for the fluorescent substrate within the respective immune cell population. n: number of biological replicates per group. Median values are calculated for each group. MFI is the geometric mean of the Mean Fluorescence Intensity (here gated on the LacZ+ subpopulation of the respective cell type).Analysis of Ifi27l2a-LacZ reporter gene expression by flow cytometry.