Sciatic nerve crush regulation of RNA alternative poly-adenylation

Poly-adenylation regulates post-transcriptional processing and localization of mRNA. To study the effect of nerve injury on poly-adenylation across the transcriptome, 3'-end RNAseq was carried on DRG neurons following sciatic nerve injury. Mice were subjected to crush of the sciatic nerve in one side, while the other nerve was spared and used as naive control condition. Mice were subjected to crush injury on in one sciatic nerve. Corresponding DRG (Lumbar L4-L6) were extracted 3 days after injury. Injured and naive ganglia was extracted from each animal. 4 mice (replicates) were used.