Photochemical decomposition rates of pteridines and flavins in seawater from Rib Reef, Great Barrier Reef

Photochemical decomposition rates of pteridines and flavins in seawater from Rib Reef were determined by reverse-phase liquid chromotography with fluorometric detection. The water was exposed to ambient surface solar radiation and samples removed at various time intervals [?2 hr before noon, noon, 2 hr after noon?] and direct injection (10-100µl) HPLC analyses conducted. For each component, the fluorescent transformation products were identified along with quantity, and the first-order decomposition rates (sea water, distilled water, distilled water with Cu2+ ion) measured.
To examine the ecological significance of the occurrence, influence and fate of pteridines and flavins in coral reef waters.
Pteridines: 6-biopterin, 6-hydroxymethylpterin, folic acid, isoxanthopterin, pterin, pterin-6-carboxylic acid, xanthopterin.Flavins: lumichrome, lumiflavin, riboflavin, riboflavin-5-phosphate.

本数据集采用反相液相色谱-荧光检测法（reverse-phase liquid chromatography with fluorometric detection），测定了取自里布礁（Rib Reef）海域海水中蝶啶类（pteridines）与黄素类（flavins）的光化学分解速率。实验中将水样暴露于自然地表太阳辐射，在正午前2小时、正午、正午后2小时等多个时间间隔采集样品，随后以10-100μl的进样体积直接进样，进行高效液相色谱（High Performance Liquid Chromatography，简称HPLC）分析。针对每一种目标组分，本研究鉴定了其荧光转化产物并测定了其含量，同时测定了三种体系中的一级分解速率，三种体系分别为天然海水、蒸馏水及添加Cu²+离子的蒸馏水。
本研究旨在探究蝶啶类与黄素类在珊瑚礁海域中的存在、影响与归趋的生态学意义。
蝶啶类组分包括：6-生物蝶呤（6-biopterin）、6-羟甲基蝶呤（6-hydroxymethylpterin）、叶酸（folic acid）、异黄蝶呤（isoxanthopterin）、蝶呤（pterin）、蝶呤-6-羧酸（pterin-6-carboxylic acid）、黄蝶呤（xanthopterin）。
黄素类组分包括：卢米黄素（lumichrome）、光黄素（lumiflavin）、核黄素（riboflavin）、核黄素-5-磷酸（riboflavin-5-phosphate）。