Gene expression data of four cancer cell lines (A375, A549, MCF7, and PC3) treated with KR-37524

KR-37524, known as a Urotensin-II receptor antagonist, has low usability due to its low affinity. For this reason, gene expression information was obtained by treating KR-37524 in four cell cancer cell lines to find a new target. The change in gene expression profile was measured and compared with the LINCS L1000 database and used to find a new target. Six sheets of each cell line were seeded in a 60mm dish and incubated for 24 hours. After 24 hours, DMSO and KR-37524 were diluted 1000-fold in medium and the cells were treated. After 6 hours, the medium was removed and cells were obtained by treatment with 1ml of TRIzol. Cells were disrupted by pipetting and stored in a deep freezer.