Selected RE recognition sites with compatible cohesive ends.
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Four pairs of RE recognition sites with compatible cohesive ends were suitable for the plasmid used in this study. The 4 pairs were divided into 2 ‘core’ sets so that the enzymes with the most similar buffer requirements were grouped together, based on the % activity of each enzyme in the 4 different New England Biolabs buffers plus standard PCR buffer (catalog & Technical Reference, 2007–08). *Bsi WI was optimally active at 55°C and 50 % active at 37°C.
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2015-12-02
